A Biomimetic Polynucleotides–Hyaluronic Acid Hydrogel Promotes Wound Healing in a Primary Gingival Fibroblast Model

Polynucleotides (PN) have long been known as an effective supportive therapy for wound healing. The present study investigated whether a hydrogel formulation containing PN and hyaluronic acid (PN + HA) could promote healing in vitro model of gingival fibroblasts. promoted cell growth viability assessed by different assays, HA, though not significantly further increasing compared to PN, supported the formation dense multilayered nodules. fibroblasts’ clonogenic efficiency HA enhanced more numerous colonies. appeared increase expression collagen 1a1 3a1, while affecting proteoglycans deposition. Interestingly, when tested scratch assay, achieved gap closure after 48 h, cells comparison groups had completely bridged even 96 h. Taken together, these results demonstrate that is promising candidate soft tissue oral cavity.